Invitrogen Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 647, Invitrogen™

Description

To minimize cross-reactivity, the goat anti-human IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, mouse, and rabbit to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment to eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. This antibody binds to heavy chains on human IgG and light chains on all human immunoglobulins. This antibody does not bind non-immunoglobulin human serum proteins or serum proteins/IgG from bovine, mouse, and rabbit.