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Gibco™ Human EGF Recombinant Protein

Product Code. p-5232662
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Quantity:
1 mg
10 μg
100 μg
25 μg
Unit Size:
Each
Product Code. Quantity unitSize
10533084 100 μg Each
10628523 1 mg Each
10193743 10 μg Each
10768225 25 μg Each
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Product Code. 10533084

Brand: Gibco™ PHG0311

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Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, PBS buffer to a concentration of 0.1-1.0 mg/mL. Make any further dilutions of the reconstituted protein in low endotoxin medium, or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA Storage: Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01133
For Use With (Application) Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 1950
Molecular Weight (g/mol) 6.2 kDa
Name Human EGF
Purification Method Purified
Quantity 100 μg
Storage Requirements 4°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
Common Name EGF
Gene Symbol EGF
Biological Activity ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Content And Storage 4°C
Classification Carrier-Free
Activity ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells.
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Expression System E. coli
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype EGF (Epidermal Growth Factor)
Research Category Stem Cell Research, Oncology
Product Line Gibco
Purity or Quality Grade >95% by SDS-PAGE
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