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HLA-ABC Monoclonal Antibody (W6/32), Super Bright™ 436, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 62-9983-41
Additional Details : Weight : 0.01000kg
Description
Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis. Applications Tested: This W6/32 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions.
Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. maligt cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.Specifications
HLA-ABC | |
Monoclonal | |
5 μL/Test | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
P05534 | |
Mouse | |
25 Tests | |
Primary | |
Human | |
Antibody | |
IgG2a κ |
Flow Cytometry | |
W6/32 | |
Super Bright 436 | |
HLA-A | |
HLA-A | |
Affinity chromatography | |
RUO | |
3105 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |