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Granzyme B Monoclonal Antibody (16G6), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
14440.00 NOK - 36070.00 NOK
Specifications
Antigen | Granzyme B |
---|---|
Clone | 16G6 |
Concentration | 0.5 mg/mL |
Applications | ELISA, Immunohistochemistry (Paraffin), Western Blot |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
17108585
|
Affymetrix eBioscience
14-8822-37 |
2 mg |
14440.00 NOK
2mg |
Estimated Shipment: 27-05-2024 Log in to see stock. |
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17118585
|
Affymetrix eBioscience
14-8822-95 |
10 mg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
The 16G6 antibody reacts with mouse Granzyme B (GrB) which is a member of the granzyme serine protease family. GrB is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis. For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (cat. 00-5523). Other buffers may yield varying results. For more information, please contact technical support at tech@ebioscience.com.
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.Specifications
Granzyme B | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
IgG2b κ | |
Affinity chromatography | |
Antibody |
16G6 | |
ELISA, Immunohistochemistry (Paraffin), Western Blot | |
Unconjugated | |
Rat | |
PBS with 0.09% sodium azide; pH 7.2 | |
Primary | |
4° C | |
GZMB |