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Gold nanoparticles, 20nm, supplied in 0.1mM PBS, 95%, OD1, 524 nm absorption
Description
For development of peptide and protein gold conjugates for use in applications such as blotting, microscopy and TEM
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
- For development of peptide and protein gold conjugates for use in applications such as blotting, microscopy and TEM
Specifications
Specifications
| Color | Red |
| Quantity | 100 mL |
| Particle Size | 20nm |
| Sensitivity | Light sensitive |
| Form | Liquid |
| Percent Purity | 95% |
| Chemical Name or Material | Gold nanoparticles, supplied in 0.1mM PBS, 95%, OD: 1, 524 nm absorption |
Frequently Asked Questions (FAQs)
We recommend using immunoblotting (dot-blot) to test a gold conjugate for the presence of the conjugated antibody (or antigen). Another alternative is to directly use the gold conjugate in the downstream application and compare its performance to a negative control gold conjugate.
The normal color of the gold nanoparticles is red. Due to repulsive forces arising from the surface charge of gold nanoparticles, an energy barrier must be overcome for individual particles to interact. When no, or a small, amount of electrolytes such as NaCl are present, this energy barrier is too strong for interaction to occur between particles. However, once NaCl is added, this energy barrier is reduced and allows the gold nanoparticles to interact and aggregate. This aggregation causes a phenomenon called "surface-plasmon coupling" which changes the adsorption maximum of light to a higher wavelength, resulting in a change in color of the solution.
Gold nanoparticles should be stored at 4-25° Celsius. Do not store gold nanoparticles in the freezer since this cause irreversible aggregation.
Settling of gold nanoparticles on the bottom of the bottle is normal and is especially common for larger sized particles, which settle at a greater speed. Settling does not affect the performance of the particles. Prior to use, we recommend swirling the solution to properly disperse your gold nanoparticles.
For most applications, our gold nanoparticles can be used without any additional washing steps. If you have a sensitive application that requires additional washing, we recommend centrifuging the gold nanoparticles. The easiest way to remove possible contaminants in the nanoparticle solution is by centrifugation. We recommend the following protocol:
- Place 1 mL aliquot of colloidal gold in a 1.5 mL micro centrifuge tube.
- Centrifuge the gold nanoparticles for 30 minutes at 10,000 RPM.
- Remove the supernatant and re-suspend in an appropriate volume of ultra-pure water.
- Vortex to re-disperse the particles.
RUO – Research Use Only
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