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  6. Invitrogen™ Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 647

Invitrogen™ Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 647

Product Code. 17629306
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Quantity:
50 μL
250 μL
Unit Size:
250µL
50µL
Product Code. Quantity unitSize
17629306 250 μL 250µL
17639306 50 μL 50µL
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Product Code. 17629306

Brand: Invitrogen™ A55060

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Description

Goat Recombinant Superclonal Secondary Antibody

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity and superior lot-to-lot consistency. Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed. Obtain superior images with the new Invitrogen Alexa Fluor Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor Plus secondary antibodies provide brighter signal compared to leading Alexa Fluor secondary antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgG (H+L)
Applications Flow Cytometry, Western Blot, Immunocytochemistry
Classification Recombinant Superclonal
Concentration 1 mg/mL
Conjugate Alexa Fluor Plus 647
Formulation Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.5
Host Species Goat
Immunogen Purified Mouse Immunoglobulin.
Purification Method Affinity chromatography
Quantity 250 μL
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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