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Gibco™ Ham's F-12K (Kaighn's) Medium
Description
Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.
This F-12K (Kaighn's) Medium is manufactured as follows:
With: L-glutamine, Phenol Red
Without: HEPES
The complete formulation is available.
Using Ham's F-12K
Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Cell Type | Human Hepatocytes |
| Classification | Animal Origin-free |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | Ham's F-12K (Kaighn's) Medium |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate |
| Without Additives | No HEPES |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
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For Research Use Only. Not for use in diagnostic procedures.
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