missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10686404

Thermo Scientific™ GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells

Product Code. 10686404
Click to view available options
Quantity:
2 x 106 Cells
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10686404

Brand: Thermo Scientific™ K1749

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession No. NP_037477) stably integrated into the CellSensor™ NFAT-bla Freestyle HEK 293F cell line.

GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession No. NP_037477) stably integrated into the CellSensor™ NFAT-bla Freestyle HEK 293F cell line. CellSensor™ NFAT-bla Freestyle HEK 293F cells (Cat. no. K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element.

The GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells are functionally validated for Z' and EC50 concentrations of dDAVP. In addition, GeneBLAzer™ GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

G2A is an orphan GPCR. Upon overexpession G2A is constitutively active. This is an assay for the orphan GPCR based upon constitutive activity. To use the assay to detect inverse agonists, high amounts of doxycycline should be added. To use the assay to detect agonists, low amounts of doxycycline should be added.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_013345
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells
• GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol G2A
Includes GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells, GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F Assay Protocol, Certificate of Analysis
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry GPR132, G2A
Type Dividing Cells
Cell Line FreeStyle 293-F Cells
System Type GeneBLAzer™
Druggable Target GPCR
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.