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Thermo Scientific™ GeneBLAzer™ Gα15-NFAT CHO-K1 DA Assay Kit
Description
The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 (Cat. No. K1078) cell line. NFAT-bla CHO-K1 cells contain a beta-lactamase reporter gene under control of an NFAT response element stably integrated into CHO-K1 cells. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.
Specifications
Specifications
| Cell State | Division-Arrested Cells |
| Content And Storage | Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ Gα15-NFAT CHO-K1 Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
| Detection Method | Fluorescence |
| For Use With (Application) | GPCRs |
| Gene Symbol | Gα15 |
| Product Line | GeneBLAzer |
| Quantity | 1 Plate |
| Reporter Gene | BLA (Beta-Lactamase) |
| Type | Division-Arrested Cells Assay Kit |
| Cell Line | CHO-K1 |
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For Research Use Only. Not for use in diagnostic procedures.
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