missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10465575

Thermo Scientific™ GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit

Product Code. 10465575
Change view
Click to view available options
Quantity:
1 Plate
Unit Size:
Each
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10465575 1 Plate Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10465575 Supplier Thermo Scientific™ Supplier No. K1577

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

The GeneBLAzer™ ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

The GeneBLAzer™ ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession No. NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element.

Both ADRA1A CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000680
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ ADRA1A CHO-K1DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ ADRA1A CHO-K1Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ ADRA1A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Description GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, GeneBLAzer™ System, Liquid Nitrogen Storage
Detection Method Fluorescence
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Gene Symbol ADRA1A
Includes Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ ADRA1A CHO-K1DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, GeneBLAzer™ ADRA1A CHO-K1Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
Population Status Pool of Stable Cells
Product Line GeneBLAzer
Quantity 1 Plate
Reporter Gene BLA (Beta-Lactamase)
Sample Type CHO-K1
Target Entry ADRA1A
Type Division-Arrested Cells Assay Kit
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
Afficher plus Afficher moins

For Research Use Only. Not for use in diagnostic procedures.

Nom du produit
Sélectionnez un problème

En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.