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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit
Description
Includes
- CRISPR OFP Nuclease Vector, linearized
- 10X Oligonucleotide Annealing Buffer
- DNase/RNase-free Water
- 5X Ligation Buffer
- T4 DNA Ligase
- U6 Forward Sequencing Primer
- Control Double-stranded Oligonucleotide
Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells. The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21178).
The GeneArt™ CRISPR Nuclease Vector system offers:
• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell lines
For research use only. Not for use in diagnostic procedures.
The GeneArt™ CRISPR Nuclease Vector system offers:
• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell lines
For research use only. Not for use in diagnostic procedures.
Certifications
CoA
Compliance
CoA
Recommended Storage
Store in freezer (-5 to -30°C)
Specifications
Specifications
| Cloning Method | Restriction Enzyme/MCS |
| Content And Storage | Contains: • CRISPR OFP Nuclease Vector, linearized • 10X Oligonucleotide Annealing Buffer • DNase/RNase-free Water • 5X Ligation Buffer • T4 DNA Ligase • U6 Forward Sequencing Primer • Control Double-stranded Oligonucleotide Store in freezer (-5 to -30°C). |
| Detection Method | Primer-probe |
| Form | Liquid |
| Format | Kit |
| Reaction Speed | Fast |
| Technique | CRISPR-Cas9 |
| Product Line | GeneArt |
| Product Type | Crispr Nuclease Vector With ofP Reporter Kit |
| Promoter | U6, CMV |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
Product Title
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