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GDF3 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Description
AA Sequence of recombinant protein: AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDF-3. Anti-Human GDF-3-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hGDF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDF-3 (500-P235) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGDF-3. Western Blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGDF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P235BT-1MG will be provided as 2 x 500 μg
Specifications
Specifications
| Antigen | GDF3 |
| Applications | ELISA, Western Blot |
| Classification | Polyclonal |
| Concentration | 0.1-1.0 mg/mL |
| Conjugate | Biotin |
| Formulation | PBS with no preservative |
| Gene | GDF3 |
| Gene Accession No. | Q9NR23 |
| Gene Alias | C78318; ecat9; Gdf3; Gdf-3; growth differentiation factor 3; growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; RGD1564178; UNQ222/PRO248; VG-1-related protein 2; Vgr2; Vgr-2 |
| Gene Symbols | GDF3 |
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