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Invitrogen™ Gateway™ pENTR™ 1A Dual Selection Vector Product Code.: 10618113

Invitrogen™ Gateway™ pENTR™ 1A Dual Selection Vector

Product Code. 10618113
10 μg, Each
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Quantity:
10 μg
Unit Size:
Each
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24113” to get your promotional price

Product Code. 10618113

Brand: Invitrogen™ A10462

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This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24113” to get your promotional price

Gateway™ pENTR™ 1A Dual Selection Vector

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:

  • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
  • Kozak consensus sequence for efficient translation initiation in eukaryotic systems
  • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
  • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
  • pUC origin for high-copy replication and maintenance of the plasmid in E. coli
  • Kanamycin resistance gene for selection in E. coli
  • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
    • o negative selection and
    • o Chloramphenicol selection in E. coli
  • Kanamycin resistance gene for selection in E. coli
TRUSTED_SUSTAINABILITY

Specifications

Product Type Dual SelectionExpression Vector
Content And Storage 10 μg pENTR™ Dual Selection vector, lyophilized in TE, pH: 8.0. Resuspend in water
Store at -20°C
Antibiotic Resistance Bacterial Chloramphenicol (CmR), Kanamycin (KanR)
Cleavage No Cleavage Site
Protein Tag Untagged
Cloning Method Gateway
Quantity 10 μg
Vector pENTR
Product Line pENTR™

Manuals

For Research Use Only. Not for use in diagnostic procedures.

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