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Anti-Mouse/Rat Foxp3 Staining Set APC, eBioscience™
Antibody Staining Kit
Brand: Invitrogen 77-5775-40
Includes: Fixation/Permeabilization Concentrate (4x): 30mL. Store at 2-8°C. Avoid agitation. Dilute 1 part Fixation/Permeabilization Concentrate with 3 parts Fixation/Permeabilization Diluent to make the Fixation/Permeabilization working solution. Caution: Wear proper protective clothing and gloves.
Fixation/Permeabilization Diluent: 100mL. Store at 2-8°C. The diluent is intended to be used in combination with the Fixation/Permeabilization Concentrate.
Permeabilization Buffer (10X): 100mL. Store at 2-8°C. Dilute to 1X with deionized/distilled water and store at 4°C. Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution.
anti-mouse/rat Foxp3 (FJK-16s): 25μg. Store at 2-8°C in the dark.
Description
The FJK-16s antibody reacts with mouse, rat, dog, porcine, and bovine Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.
Intracellular staining of mouse splenocytes with FJK-16s using the PE anti-mouse/rat Foxp3 Staining Set and protocol reveals approximately 2% of total cells in the C57Bl/6 strain and approximately 3-5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+CD25+ cells and 4% of the CD4+CD25- cells staining with FJK-16s. B220+, CD11b+, CD11c+, and Ly6G/Gr-1+ cells do not show significant co-staining with FJK-16s.
Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.
Not included:
Fc Block (cat. 14-0161)
Flow Cytometry Staining Buffer (cat. 00-4222)
Rat IgG2a isotype Control (cat. 12-4321, 11-4321, or 17-4321)
Specifications
| Foxp3 | |
| Monoclonal | |
| 0.2mg/mL | |
| Rat | |
| RUO | |
| Mouse, Rat | |
| IgG2a κ |
| Flow Cytometry | |
| FJK-16s | |
| APC | |
| 1 kit | |
| Primary | |
| Store at 2-8°C. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle. | |
| eBioscience™ |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Anti-Mouse/Rat Foxp3 Staining Set APC, eBioscience
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 1A
- Germ cell mutagenicity Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity after single exposure Category 1
Hazard Statement
- H317-May cause an allergic skin reaction.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
- H370-Causes damage to organs.
Precautionary Statement
- P201-Obtain special instructions before use.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Formaldehyde, methanol
Product Content Correction
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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