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FOXP3 Monoclonal Antibody (236A/E7), PE-Cyanine5, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
2125.00 NOK - 4625.00 NOK
Specifications
Antigen | FOXP3 |
---|---|
Clone | 236A/E7 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
16506930
|
Affymetrix eBioscience
15-4777-42 |
100 Tests |
4625.00 NOK
Pack of 100 |
In Stock Log in to see stock. |
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16596920
|
Invitrogen
15-4777-41 |
25 Tests |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Description: The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25^bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). Applications Reported: This 236A/E7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 236A/E7 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol.
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.Specifications
FOXP3 | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID | |
FOXP3 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
FOXP3 |
236A/E7 | |
Flow Cytometry | |
PE-Cyanine5 | |
Mouse | |
Human, Non-human Primate, Rhesus Monkey | |
Q9BZS1 | |
50943, 574303 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |