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Flag tag Rabbit anti-Tag, Polyclonal, Proteintech

Product Code. 16850095 Shop All Proteintech Products
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Quantity:
150 μL
Unit Size:
150µL
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16850095 150 μL 150µL
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Product Code. 16850095 Supplier Proteintech Supplier No. 205431AP150UL

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Description

Rabbit Polyclonal Antibody

This antibody is a rabbit polyclonal antibody raised against the 3xDYKDDDDKT sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.

DDK (FLAG-tag, FLAG octapeptide, FLAG epitope) is a polypeptide protein tag that can be added to a protein using recombit DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. It has been used to separate recombit, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.

Specifications

Antigen Flag tag
Applications Immunocytochemistry, Immunofluorescence, Western Blot
Classification Polyclonal
Concentration 0.24 mg/mL
Conjugate Unconjugated
Formulation PBS with 50% glycerol and 0.1% sodium azide; pH 7.3
Gene Alias DYKDDDDK, DDDDK, DDDDK Tag
Host Species Rabbit
Immunogen DDDDK Tag Fusion Protein Ag2329
Purification Method Antigen Affinity Chromatography
Quantity 150 μL
Regulatory Status RUO
Primary or Secondary Primary
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG
Target Molecule Tag
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