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Flag tag Mouse anti-Tag, Clone: 2B3C4, Proteintech

Artikelnummer. 16807505 Shop alle Proteintech produkter
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Quantity:
150 μL
Pakningsstørrelse:
150µL
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16807505 150 μL 150µL
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Artikelnummer. 16807505 Leverandør Proteintech Leverandørnr. 660083IG150UL

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Mouse Monoclonal Antibody

Flag Tag Antibody is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, independently on N-terminal, C-terminal or internal regions of the target protein.

DDK (FLAG-tag, FLAG octapeptide, FLAG epitope) is a polypeptide protein tag that can be added to a protein using recombit DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. It has been used to separate recombit, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.

Tekniske data

Antigen Flag tag
Applications Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Classification Monoclonal
Clone 2B3C4
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 50% glycerol and 0.1% sodium azide; pH 7.3
Gene Alias DYKDDDDK, DDDDK, DDDDK tag
Host Species Mouse
Immunogen Peptide
Purification Method Protein G
Quantity 150 μL
Regulatory Status RUO
Primary or Secondary Primary
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Tag
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