missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. Thermo Scientific™ SatI (Fnu4HI) (10 U/μL)

Thermo Scientific™ SatI (Fnu4HI) (10 U/μL)

Product Code. 10764501 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
1,000 units
200 units
Unit Size:
1000 units
200 units
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10764501 1,000 units 1000 units
10558170 200 units 200 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 10764501 Supplier Thermo Scientific™ Supplier No. ER1642

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).

Lambda DNA digested with SatI (Fnu4HI), 1.4% agarose, 380 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G C▵N G C...3'

3'...C G N▵C G...5'

Conditions for 100% Activity

  • 1X Buffer G:10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • SatI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with SatI, more than 60% of the DNA fragments can be ligated in a reaction mixture containing 10-30U of T4 DNA Ligase/1μg of fragments and 10% PEG
  • More than 90% of these can be recut

Methylation Effects

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Compatible Ends

  • GC^SGC — BcnI, Bme1390I
  • GC^WGC — MvaI, Bme1390I

Note:

At least two copies of SatI (Fnu4HI) recognition site are required for efficient cleavage.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 U/μL
Methylation Sensitivity CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme SatI (Fnu4HI)
Compatible Buffer 10X Buffer G
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 1,000 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.