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Thermo Scientific™ X-Gal Solution, ready-to-use Product Code.: 10508750
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Thermo Scientific™ X-Gal Solution, ready-to-use

Product Code. 10508750 Shop All Thermo Scientific Products
10 mL, 10mL
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Quantity:
10 mL
Unit Size:
10mL
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24113” to get your promotional price

Product Code. 10508750

Brand: Thermo Scientific™ R0941

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This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24113” to get your promotional price

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Formula: C14H15BrClNO6 Molecular weight: 408.6

X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).

X-Gal Solution, ready-to-use, is stable, 0.22 ÎĽm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies. X-Gal Solution, ready-to-use, is a stable, 0.22ÎĽm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Recommended for:

Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures.

Note:

Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF-containing solution to plastic Petri dishes should be avoided. DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.

TRUSTED_SUSTAINABILITY

Specifications

Detection Method Colorimetric
Product Type X-Gal Solution
Quantity 10 mL
Substrate Properties Chemical Substrate
Substrate Type Beta-Gal Substrate, X-Gal

Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number2796035 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeR0941
Lot Number2822302 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeR0941
Lot Number2855266 Certificate TypeCertificate of Analysis Date28/01/2025 Product CodeR0941
Lot Number2873608 Certificate TypeCertificate of Analysis Date28/01/2025 Product CodeR0941
Lot Number2906597 Certificate TypeCertificate of Analysis Date28/01/2025 Product CodeR0941
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Product Identifier
  • X-Gal Solution, ready-to-use
Signal Word
  • Warning
Hazard Category
  • Flammable liquid Category 3
  • Skin sensitiser Category 1
Hazard Statement
  • H226-Flammable liquid and vapour.
  • H317-May cause an allergic skin reaction.
Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P233-Keep container tightly closed.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P370+P378-In case of fire: Use dry sand, dry chemical, or alcohol-resistant foam to extinguish.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
  • MIXTURE LIST-Contains : ethanol, hydroquinone, DMSO
  • Multiple Manufacturers
    Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    Promo Code: 24158

    Expires2025-10-31

    Days

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    Minutes

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    *Discount will apply to qualifying orders received by Fisher Scientific between 01/09/2025 and 31/10/2025. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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For Research Use Only. Not for use in diagnostic procedures.

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