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Thermo Scientific™ GeneJET Plant RNA Purification Kit
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Requires
Dithiothreitol (DTT, #R0861)
Thermo Scientific GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.
Highlights
- Fast—procedure completed in 20 min following cell lysis
- Convinient—protocol does not include clogging-prone lysate filtration step
- High yield—up to 65 μg of high purity and high integrity RNA from 50 mg plant sample
- Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants)
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- RT-PCR
- RT-qPCR
- Northern blotting
- Nuclease protection assay
Especificaciones
Especificaciones
| Content And Storage | • 200 mL Plant RNA Lysis Solution; store at room temperature • 200 mL Wash Buffer WB 1 (concentrated); room temperature • 2 x 100 mL Wash Buffer 2 (concentrated); room temperature • 125 mL Water, Nuclease-free; room temperature • 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C. • 250 Collection Tubes (2 mL); room temperature • 250 Collection Tubes (1.5 mL); room temperature |
| Isolation Technology | Silica Spin Column |
| Purification Time | 20 min. (After sample homogenization) |
| Elution Volume | 50 μL |
| Sample Type | Plants |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
| Quantity | 250 Preps |
| Scale | Mini |
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For Research Use Only. Not for use in diagnostic procedures.
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