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Thermo Scientific™ GeneJET Plant Genomic DNA Purification Kit
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GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
Highlights
• Fast—procedure completed in 30 min following cell lysis
• Convenient—protocol does not include clogging-prone lysate filtration step
• High yield—up to 32 μg of high quality DNA from 100 mg sample, including nuclear, mitochondrial, and chloroplast DNA
• Compatible—use with awide variety of samples (leaves, roots, sprouts and other parts of various plants)
The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (e. g. young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions.
Applications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting
Specifications
Specifications
| Column Type | Spin Column |
| Content And Storage | • 6 x 1 mL RNase A; store at room temperature until opened, –20°C after opening • 120 mL Lysis Buffer A; room temperature • 15 mL Lysis Buffer B; room temperature • 40 mL Precipitation Solution; room temperature • 120 mL Plant gDNA Binding Solution; room temperature • 40 mL Wash Buffer I (concentrated); room temperature • 40 mL Wash Buffer II (concentrated); room temperature • 150 mL Elution Buffer; room temperature • 250 GeneJET Purification Columns with Collection Tubes; room temperature • 250 Collection Tubes; room temperature |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Plants |
| Elution Volume | 200 μL |
| Final Product Type | gDNA |
| For Use With (Application) | PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Quantity | 250 Preps |
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Safety and Handling
- GeneJET Plant Genomic DNA Purification Kit
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sodium lauryl sulfate, Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.
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