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Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit
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Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.
Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.
- Non-radioactive labeling of DNA
- Efficient priming of labeling reactions with random decamers
- High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction
Recommended for:
Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations
Specifications
Specifications
| Detection Method | Biotin-based |
| Format | Kit |
| Final Product Type | DNA |
| Labeling Target | Genomic DNA, PCR Products, DNA (General) |
| Includes Label or Dye | Yes |
| Labeling Method | Direct Labeling |
| No. of Reactions | 10 |
| Product Line | DecaLabel |
| Product Type | DNA Labeling Kit |
| Quantity | 10 Reactions |
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