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  5. Thermo Scientific™ BseDI (BsaJI) (10 U/μL)

Thermo Scientific™ BseDI (BsaJI) (10 U/μL)

Product Code. 10689330 Shop All Thermo Scientific Products
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Product Code. 10689330 Supplier Thermo Scientific™ Supplier No. ER1081

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Description

Cut at C^CNNGG sites with BseDI (BsaJI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BsaJI, BssECI).

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C▵C N N G G...3'

3'...G G N N C▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • BseDI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BseDI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlap — no effect
  • Dcm: may overlap — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Note:

Incubation at 37°C results in 10% activity.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme BseDI (BsaJI)
Compatible Buffer 10X Buffer Tango
Sensitive to Heat Inactivation No
Optimal Reaction Temperature 55°C
Type IIS RE No
Quantity 300 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

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