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Thermo Scientific™ BCIP-T

Product Code. 10765081 Shop All Thermo Scientific Products
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Product Code. 10765081 Supplier Thermo Scientific™ Supplier No. R0821

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Description

A chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium, †R0841) to enhance blue color development.

Thermo Scientific™ Fermentas BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium, †R0841) to enhance blue color development.

Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan (1)

Recommended for:

Detection of alkaline phosphatase activity in:

  • Southern blotting
  • Western blotting
  • Northern blotting
  • dot/slot blots
  • plaque and colony screening

Note:

Preparation of a 50mg/mL stock solution in dimethylformamide is recommended.

TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Detection of alkaline phosphatase activity in:; Southern blotting Western blotting Northern blotting dot/slot blots plaque and colony screening;
Product Type BCIP-T
Quantity 1 g
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