F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488, Invitrogen™

Description

F(ab')2 antibody fragments offer several benefits over whole IgGs, due to their smaller size (∽110 kD vs. ∽ 150 kD) for better penetration into tissue sections, resulting in improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells with Fc receptors (such as immune cells) and other Fc interaction-related interference. F(ab')2 antibody fragments were obtained by a combination of pepsin digestion and immunoaffinity chromatography to remove residual Fc fragments and undigested whole IgG molecules. To minimize cross-reactivity, the goat anti-rabbit IgG F(ab')2 fragments have been cross-adsorbed against human, mouse and rat serum proteins to increase specificity of the antibody resulting in higher sensitivity and less background staining. This antibody binds to heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins, does not bind to human, mouse or rat serum proteins/IgG or rabbit non-immunoglobulin serum proteins.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.