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  6. Invitrogen™ Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), eBioscience™, Invitrogen™

Invitrogen™ Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), eBioscience™, Invitrogen™

Product Code. 15217447
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Product Code. 15217447 Supplier Invitrogen™ Supplier No. 14574185

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Description

Mouse Monoclonal Antibody

Description: The Y-Ae antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules. The Y-Ae antibody detects a determit expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This determit is expressed on peripheral B cells and on cells in the thymic medulla, but not thymic cortical epithelium. In addition to B cells, the Y-Ae determit is expressed at comparable levels on other antigen presenting cells, including macrophages and dendritic cells. The antibody does not react with invariant chain-associated class II MHC complexes. The reactivity of the Y-Ae antibody is MHC-restricted. The Y-Ae antibody was generated by immunization of [B10.MBR x B10.D2]F1 recipients with B10A(5R) LPS blasts. In this strain combination, there is incompatibility for I-Ab and I-Eb molecules. The antibody reacts with 10-15% of surface I-Ab molecules in strains that also express surface I-Eb molecules (e.g., strains B10.A(5R) and B10.A(3R)). The antibody does not react with I-A molecules in strains that have a non-functional I-E (a chain) gene (e.g., strains B6 and B10), nor with mutant I-A molecules (e.g., (B6.c-H-2bm-12 x A.TFR5)F1), nor with any other strain tested. The Y-Ae antibody recognizes an Ea peptide (Ea 52-68) bound to I-Ab molecules, but not to Ea 52-68 peptide bound to I-Ak or I-Abm-12 molecules.

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Ea52-68 peptide bound to I-Ab
Applications Flow Cytometry
Classification Monoclonal
Clone eBioY-Ae (YAe, Y-Ae), Y-Ae)
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene H2-Ab1
Gene Accession No. P01921, P06342, P06343, P06344, P06345, P06346, P14483
Gene Alias Abeta; AI845868; H-2 class II histocompatibility antigen, A beta chain; H-2Ab; H2-Ab; H2-Ab1; H2Eb; H-2Eb; H2-iabeta; histocompatibility 2, class II antigen A, beta 1; Ia2; Ia-2; Ia4; Ia-4; IAb; I-Abeta; major histocompatibility complex class II beta chain; MGC163794; MGC163796; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1; Rmcs1
Gene Symbols H2-Ab1
Host Species Mouse
Purification Method Affinity chromatography
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 14961
Target Species Mouse
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2b
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