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  5. IBA Lifesciences pASG-IBA E. coli Expression Vector with Secretion

IBA Lifesciences pASG-IBA E. coli Expression Vector with Secretion
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Product Code. 15673748 Shop All IBA Lifesciences Products
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Vector:
pASG-IBA102
pASG-IBA104
pASG-IBA144
pASG-IBA2
pASG-IBA4
pASG-IBA44
pASG-IBAwt2
Protein Tag:
His Tag (6x), Strep-tag II
His Tag (6x), Twin-Strep-tag
Strep-tag II
Twin-Strep-tag
Untagged
Unit Size:
5µg
7 product options available for selection
Product selection table with 7 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Vector Protein Tag unitSize
15673748 pASG-IBA144 His Tag (6x), Twin-Strep-tag 5µg
15613748 pASG-IBA102 Twin-Strep-tag 5µg
15633748 pASG-IBA104 Twin-Strep-tag 5µg
15643728 pASG-IBAwt2 Untagged 5µg
15643738 pASG-IBA44 His Tag (6x), Strep-tag II 5µg
15653728 pASG-IBA2 Strep-tag II 5µg
15673728 pASG-IBA4 Strep-tag II 5µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 7 options available.
7 options
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Product Code. 15673748 Supplier IBA Lifesciences Supplier No. 54144001

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This item is not returnable. View return policy

Description

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. E. coli Expression Vector with Secretion allows for the expression of proteins in E. coli.

  • The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.

  • The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.

  • The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.

  • Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.

Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Inducing Agent Inducible with anhydrotetracycline
Marker Gene Ampicillin
Promoter Tet
Product Type Vector
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein Tag His Tag (6x), Twin-Strep-tag
Quantity 5 μg
Vector pASG-IBA144
For Use With (Application) ompA for periplasmic secretion
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