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IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
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Vector:
pASG-IBA103
pASG-IBA105
pASG-IBA123
pASG-IBA143
pASG-IBA145
pASG-IBA162
pASG-IBA164
pASG-IBA167
pASG-IBA168
pASG-IBA23
pASG-IBA3
pASG-IBA43
pASG-IBA45
pASG-IBA5
pASG-IBA62
pASG-IBA64
pASG-IBAwt1
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5µg
Beschreibung
- The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
- The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
- The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
- Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Spezifikation
Spezifikation
| Inducing Agent | Inducible with anhydrotetracycline |
| Marker Gene | Ampicillin |
| Promoter | Tet |
| Product Type | Vector |
| Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Species | E-coli |
| Protein Tag | Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal, FLAG-tag |
| Cloning Method | Direct Cloning |
| Concentration | 250 ng/μL |
| Quantity | 5 μg |
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