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Description
Includes
DyNAmo Flash qPCR Mix (2X); 50X ROX dye, 25μM
DyNAmo Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results.
DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments.
- Extremely fast protocols: combined annealing and extension step of only 15 sec.
- Specific and sensitive detection of a wide range of template concentrations
- Included dUTP allows the use of UNG for prevention of carry-over contamination
- Convenient 2X master mix and optimized protocols
Specifications
Specifications
| Concentration | 2X (qPCR Mix), 50X (Dye) |
| Content And Storage | Contains: • 5 x 1 mL DyNAmo Flash qPCR Mix (2X) • 1 x 250 μL 50X ROX dye, 25μM Store at -20°C |
| Detection Method | Primer-probe |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | Tbr DNA polymerase |
| Reaction Speed | Fast |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, Eppendorf™ MasterCycler™ RealPlex, QuantStudio™, ViiA™ 7 System, MJ Chromo4, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, BioRad iQ5, BioRad iCycler iQ, Corbett RotorGene, Cepheid SmartCycler, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, StepOne™ System, MJ Opticon, Stratagene Mx3000P |
| Kit Type | DyNAmo Flash Probe qPCR Kit |
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For Research Use Only. Not for use in diagnostic procedures.
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