Invitrogen™ Dynabeads™ RNA Binding Buffer

Description

Dynabeads RNA Purification Buffer is intended for use with Dynabeads Carboxylic Acid for RNA Purification, providing a simple, scalable, and automation-ready solution for purification of crude RNA produced by in vitro transcription (IVT). The combination is particularly suitable where flexibility and high purity are required, such as in discovery phase of vaccine development and following the solid-phase IVT reaction with Dynabeads Streptavidin for In Vitro Transcription.

This buffer combined with Dynabeads Carboxylic Acid for RNA Purification offer efficiency, scalability (micrograms to grams of mRNA) in manual and automated workflows, and quality to navigate from RNA-based drug discovery to commercial manufacturing without incurring extra time or costs from switching technology or raw material.

Crude RNA from an IVT reaction is mixed with the beads and the buffer is added. The RNA binds to the bead surface and the remaining components of the reaction mixture are removed by applying a magnet and discarding the supernatant. Scripts are available for automated purification workflows with KingFisher systems.

The buffer is offered here as a stand-alone product. It is also included in the kits below:
Dynabeads RNA Purification Kit (Cat. No. 65030D)
Dynabeads In Vitro Transcription and RNA Purification Kit (Cat. No. 65034D)

To learn more about other products and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics visit: thermofisher.com/dynabeadsmrna