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  5. Thermo Scientific™ DpnI (10 U/μL)

Thermo Scientific™ DpnI (10 U/μL)

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Product Code. 10669500

Brand: Thermo Scientific™ ER1701

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Description

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5’G Am6↓T C 3’
3’C T ↑Am6 G 5’

Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

  • Superior quality—stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern blotting
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note: DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA. For methylation sensitivity, refer to product specifications.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 U/μL
Methylation Sensitivity Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme Dpn I
Compatible Buffer 10X Buffer Tango
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 500 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

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