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Gibco™ DMEM/F-12, no glutamine
Beschreibung
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With: Phenol Red
Without: L-glutamine, HEPES
Gibco™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.
DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Spezifikation
Spezifikation
| Cell Type | Mammalian Cells |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | Dulbecco's Modified Eagle Medium (DMEM) |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| With Additives | High Glucose, Phenol Red, Sodium Pyruvate |
| Without Additives | No Glutamine, No HEPES |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco |
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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