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Description
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
DTT has been used:
- As one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk
- As a component of medium for the demembranation and reactivation of spermatozoa
- To maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst
- As a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups
Specifications
Specifications
| CAS | 12-3-3483 |
| Melting Point | 40°C to 50°C (literature) |
| pH | 4.0 to 6.0 |
| Molecular Formula | C4H10O2S2 |
| Quantity | 250 mg |
| Synonym | dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt |
| Solubility Information | Soluble in water (50mg/mL, clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether. |
| InChI Key | VHJLVAABSRFDPM-IMJSIDKUSA-N |
| SMILES | C(C(C(CS)O)O)S |
| IUPAC Name | (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol |
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