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Collagen I Antibody, Novus Biologicals™

Product Code. 18070976 Shop All Bio Techne Products
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0.025 mg
Unit Size:
25µg
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Product Code. Quantity Unit Size
18070976 0.025 mg 25µg
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Product Code. 18070976 Supplier Novus Biologicals Supplier No. NB6004080.025MG
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Estimated Shipment: 09-09-2026
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Description

Rabbit Polyclonal Antibody has been used in 195 publications

Collagen I alpha 1 Polyclonal specifically detects Collagen I alpha 1 in Bacteria, Bovine, Chicken, Human, Mouse, Pig, Rabbit, Rat, Zebrafish samples. It is validated for ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunohistochemistry Whole-Mount, Immunoprecipitation, KnockDown, Microarray, SDS-Page, Western Blot.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Collagen I alpha 1
Applications ELISA, Fluorophore-Linked Immunosorbent Assay, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunohistochemistry (Whole-Mount), Immunoprecipitation, KnockDown, Microarray, SDS-Page, Western Blot
Classification Polyclonal
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 1:1000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:10-1:500, Immunocytochemistry 1:1600
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Accession No. P02452, P02453
Gene Alias Alpha-1 type I collagen, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, Collagen1, Collagen-1, OI4, pro-alpha-1 collagen type 1
Gene Symbols COL1A1
Host Species Rabbit
Immunogen Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.
Molecular Weight of Antigen 139 kDa
Purification Method Affinity Purified
Quantity 0.025 mg
Regulatory Status RUO
Research Discipline Cancer, Cell Cycle and Replication, Cytoskeleton Markers, Extracellular Matrix
Primary or Secondary Primary
Gene ID (Entrez) 1277
Test Specificity Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Target Species Bacteria, Bovine, Chicken, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype IgG
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