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Beschreibung
Thermo Scientific™ Clean-Blot IP Detection Reagents are HRP and AP conjugates that are optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.
Highlights:
Universal – bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
Compatible – effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
Cost-effective – eliminates the cost and extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
Flexible – available in alkaline phosphate (AP) and horseradish peroxidase (HRP) reagents for detection with chemiluminescent, fluorescent or colorimetric substrates
Easy to use – no need to change the Western blotting protocol; simply replace conventional secondary HRP conjugate with the Clean-Blot IP Detection Reagent
Unobstructed detection – clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands
Spezifikation
Spezifikation
| Description | Clean-Blot IP Detection Kit (HRP) |
| Product Type | IP Detection Reagent (HRP) |
| Detection Method | Chemiluminescence |
| Form | Liquid |
| For Use With (Application) | Western Blot |
| Product Line | Clean-Blot |
| Quantity | 25 blots |
| Conjugate | HRP |
| Reactivity | All species |
| Sufficient For | 25 Mini Blots, 2000 sq. cm Membrane |
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For Research Use Only. Not for use in diagnostic procedures.
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