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Description
Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Highlights
- Superior quality – stringent quality control and industry leading manufacturing process
- Convenient color-coded Five Buffer System
- Includes universal Tango buffer for double-digestions
- BSA premixed in reaction buffers
- Wide selection of restriction endonuclease specificities
Applications
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern Blot
- Restriction fragment length polymorphism (RFLP)
- SNP
Note
Cfr42I activity is affected by high salt concentration. Trace amounts of sodium chloride remaining in the substrate DNA after completion of upstream applications may inhibit enzyme activity and result in impaired DNA cleavage. Certain sites in lambda DNA are difficult to cleave with Cfr42I, the same as with its prototype SacII. At least two copies of Cfr42I recognition site are required for efficient cleavage.
Specifications
Specifications
| Concentration | 10 U/μL |
| Methylation Sensitivity | CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive |
| Enzyme | Cfr42I (SacII) |
| Compatible Buffer | 10X Buffer B |
| Sensitive to Heat Inactivation | Yes |
| Optimal Reaction Temperature | 37°C |
| Type IIS RE | No |
| Quantity | 5 x 1200 units |
| Product Type | Restriction Enzyme |
| Research Category | Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.
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