Learn More
Thermo Scientific™ CellSensor™ NFKB-bla Freestyle™ HEK293F Cell Line
Description
Nuclear Factor kappaB (NFκB) signaling regulates genes involved in apoptosis, viral defense, cancer, inflammation, and autoimmune disease. The CellSensor™ NFκB-bla FreeStyle™ 293F Cell Line is responsive to tumor necrosis factor alpha (TNFα) and can be used to probe the NFκB signaling pathway. This cell line was validated for DMSO tolerance, incubation time with stimulant and substrate loading conditions. The CellSensor™ NFκB-bla FreeStyle™ 293F Cell Line contains a beta-lactamase reporter gene under the control of NFκB response element. The construct was transduced into FreeStyle™ 293F cells by lentivirus. This cell line is a pool isolated for response to TNFα by flow cytometry. It responds to agonist treatment as expected from literature. Academic and non-profit customers, please inquire for special pricing.
Specifications
Specifications
| Assay Entry | Cell-based beta lactamase reporter gene |
| Cell State | Dividing Cells |
| Content And Storage | CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
| For Use With (Application) | Cytokines, Signaling Pathway |
| Product Line | CellSensor |
| Quantity | 1 Vial |
| Target Entry | TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway |
| Type | Dividing Cells |
| System Type | CellSensor™ |
| Druggable Target | Signaling Pathway |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.