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Molecular Probes™ CellROX™ Deep Red Flow Cytometry Assay Kit

CellROX™ Deep Red Flow Cytometry Assay Kit

Brand:  Molecular Probes™ C10491

Additional Details : Weight : 0.25000kg

Product Code. 15246066

  • 2905.00 NOK / 1 set


In stock, ready to ship: 7
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Beschreibung

Beschreibung

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX™ Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

  • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
  • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
  • Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

    The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
  • Spezifikation

    Spezifikation

    Fluorescent
    Contains 1 vial CellROX® Deep Red Reagent (25 µL), 1 vial SYTOX® Blue Dead Cell Stain (100 µL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water), and 1 vial DMSO (200 µL).

    Store kit at -5 to -30°C, protected from light. CellROX® reagent is air sensitive.
    633/635, UV or 405
    Flow Cytometer
    100
    Wet Ice
    Tube(s)
    Mammalian Cells, Eukaryotic Cells
    CellROX® Deep Red: 644⁄665, SYTOX® blue: 444⁄480
    CellROX® Deep Red Reagent
    DMSO (Dimethylsulfoxide)
    CellROX®
    Videos
    Dokumentation

    For Research Use Only. Not for use in diagnostic procedures.