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Molecular Probes™ CellROX™ Deep Red Flow Cytometry Assay Kit

Product Code. 15246066
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Quantity:
100 Assays
Unit Size:
1 set
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Product Code. 15246066

Brand: Molecular Probes™ C10491

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3030.00 NOK valid until 2025-12-19
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This item is not returnable. View return policy

Description

CellROX™ Deep Red Flow Cytometry Assay Kit

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX™ Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

  • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
  • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
  • Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

    The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Cell Type Mammalian Cells, Eukaryotic Cells
    Content And Storage Contains 1 vial CellROX™ Deep Red Reagent (25 μL), 1 vial SYTOX™ Blue Dead Cell Stain (100 μL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 μL, 70% solution in water), and 1 vial DMSO (200 μL).

    Store kit at -5 to -30°C, protected from light. CellROX™ reagent is air sensitive.
    Detection Method Fluorescence
    For Use With (Application) Flow Cytometry
    For Use With (Equipment) Flow Cytometer
    Product Type Reagent
    Dye Type CellROX™ Deep Red Reagent
    Emission 644/665, 444/480
    Format Tube(s)
    Product Line CellROX
    Quantity 100 Assays
    Shipping Condition Wet Ice
    Solubility DMSO (Dimethylsulfoxide)
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    Safety and Handling

    compliance-icons compliance-icons compliance-icons compliance-icons compliance-icons
    Product Identifier
    • CellROX Deep Red Flow Cytometry Assay Kit
     Signal Word
    • Danger
     Hazard Category
    • Acute toxicity Category 3
    • Acute toxicity Category 4
    • LONG-TERM AQUATIC HAZARD Chronic 2
    • Serious eye damage/eye irritation Category 1
    • Flammable liquid Category 3
    • Germ cell mutagenicity Category 2
    • Organic peroxide Type E & F
    • Skin corrosion/irritation Category 1 B
    • Skin sensitiser Category 1
     Hazard Statement
    • H226-Flammable liquid and vapour.
    • H242-Heating may cause a fire.
    • H302-Harmful if swallowed.
    • H311-Toxic in contact with skin.
    • H314-Causes severe skin burns and eye damage.
    • H317-May cause an allergic skin reaction.
    • H331-Toxic if inhaled.
    • H341-Suspected of causing genetic defects.
    • H411-Toxic to aquatic life with long lasting effects.
     Precautionary Statement
    • P201-Obtain special instructions before use.
    • P273-Avoid release to the environment.
    • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
    • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
    • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
    • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
    • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
    • P370+P378-In case of fire: Use water/water spray/water jet/carbon dioxide/sand/foam/alcoholresistant foam/chemical powder to extinguish.
     Supplemental information
    • MIXTURE LIST-Contains : tert-Butyl hydroperoxide

    For Research Use Only. Not for use in diagnostic procedures.

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