Invitrogen™ CD8a Monoclonal Antibody (53-6.7), Brilliant Violet™ 480, eBioscience™, Invitrogen™

Description

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation. Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.

CD8a Monoclonal antibody specifically detects CD8a in Mouse samples. It is validated for Flow Cytometry.