missing translation for 'onlineSavingsMsg'
Learn More

CD49d (Integrin alpha 4) Rat anti-Mouse, PE-Cyanine7, Clone: R1-2, eBioscience

Rat Monoclonal Antibody

Brand:  Invitrogen 25-0492-82

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 16596960

  • 3045.00 NOK / 100µg

Estimated Shipment: 08-11-2021

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1). Applications Reported: This R1-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
Specifications

Specifications

CD49d (Integrin alpha 4)
Monoclonal
0.2 mg/ml
PBS with 0.09% sodium azide; pH 7.2
Q00651
Itga4
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16401
Flow Cytometry
R1-2
PE-Cyanine7
Itga4
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Videos
SDS
Documents
Certificates