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  6. Invitrogen™ CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience™, Invitrogen™

Invitrogen™ CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience™, Invitrogen™

Product Code. 15236967
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Product Code. Quantity unitSize
15236967 50 μg 50µg
15246967 100 μg 100µg
15256967 500 μg 500µg
15266967 1 mg 1mg
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Product Code. 15236967

Brand: Invitrogen™ 14045281

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Description

Rat Monoclonal Antibody

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections. RA3-6B2 has also been reported in functional studies of B cells. (Please use Functional Grade purified RA3-6B2, cat. 16-0452, in functional assays.). Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RA3-6B2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low and high pH antigen retrieval and can be used at less than or equal to 5 μg/mL.

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD45R (B220)
Applications Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation
Classification Monoclonal
Clone RA3-6B2
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene PTPRC
Gene Accession No. P06800, P08575
Gene Alias B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
Gene Symbols PTPRC
Host Species Rat
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 19264, 5788
Target Species Human, Mouse
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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