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  6. Invitrogen™ CD45R (B220) Monoclonal Antibody (HIS24), eBioscience™, Invitrogen™

Invitrogen™ CD45R (B220) Monoclonal Antibody (HIS24), eBioscience™, Invitrogen™

Product Code. 17190482
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Quantity:
2 mg
10 mg
Unit Size:
10mg
2mg
Product Code. Quantity unitSize
17190482 2 mg 2mg
17100492 10 mg 10mg
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Product Code. 17190482

Brand: Invitrogen™ 14046037

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Description

Mouse Monoclonal Antibody

Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes. Applications Reported: The HIS24 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. Applications Tested: This HIS24 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD45R (B220)
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Classification Monoclonal
Clone HIS24
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene PTPRC
Gene Accession No. P04157, P08575
Gene Alias B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
Gene Symbols PTPRC
Host Species Mouse
Purification Method Affinity chromatography
Quantity 2 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 24699, 5788
Target Species Human, Rat
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG2b κ
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