CD16 Monoclonal Antibody (3G8), NovaFluor™ Yellow 610, eBioscience™, Invitrogen™

Description

This 3G8 monoclonal ab reacts with human and Non-human Primate CD16,also known aslow-affinity Fc gamma RIII. CD16 exists as two distinct isoforms, Fc gamma RIIIA and Fc gamma RIIIB. In humans, Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells. T and B cells do not express this Fc receptor. Fc gamma RIIIB is also detected on neutrophils as a GPI-anchored form. Expression of CD16 on lymphocytes and monocytes is similar in Non-human Primates. However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys. Binding of IgG leads to activation of signal transduction pathways, resulting in ab-dependent cell-mediated cytotoxicity(ADCC), phagocytosis, cytokine release, and antigen presentation.
Based on cross-blocking studies 3G8 recognizes the same epitope as CB16. However, 3G8 and B73.1 ab clones bind distinct epitopes.
Applications Reported: This 3G8 ab has been reported for use in flow cytometric analysis.
Applications Tested: This 3G8 ab has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 4uL(0.6ug) per test. A test is defined as the amount(ug) of ab that will stain a cell sample in a final volume of 100uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm(Yellow) Laser

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.