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Macherey-Nagel™ Lyophilized Carrier RNA

Product Code. 15361017
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0.3 mg
Unit Size:
0.3mg
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15361017 0.3 mg 0.3mg
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Product Code. 15361017 Supplier Macherey-Nagel™ Supplier No. 740514

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Description

Lyophilized,Carrier RNA to be used with NucleoSpin RNA XS or RNA Virus kits.

For use with RNA isolation using various NucleoSpin and NucleoMag kits: Virus, XS (extra small samples)

  • (poly(-A) RNA: poly(A) potassium salt—prepared from ADP with polynucleotide phosphorylase)
  • To prepare working solution, dissolve freeze dried Carrier-RNA in lysis buffer (e.g. RA1, RAV1)
  • Add to sample lysate (20 ng/sample)
    • Such small amounts typically do not interfere with subsequent RT-PCR, even in oligo-dT primed reverse transcriptions
    • The small amount of Carrier RNA transfered into a reverse transcription reaction does not significantly influence the outcome of the reaction, due to the large excess of oligo-dT primer
  • The benefit of adding Carrier RNA depends on sample type, amount, and kind of downstream RNA analysis
  • Carrier RNA should be omitted when, subsequent to RNA isolation, a poly-A RNA isolation / RNA sequencing is performed

Specifications

CAS 26763-19-6
Concentration 90 to <100%
Color Colorless
Content And Storage 0,3 mg, 15°C to 25°C
Formulation poly(-A) RNA: poly(A) potassium salt
For Use With (Equipment) NucleoSpin Virus and XS kits
For Use With (Application) RNA isolation for downstream applications
No. of Reactions 0.3 mg
Odor Odorless
Quantity 0.3 mg
Sample Source Prepared from ADP with polynucleotide phosphorylase
Form Lyophilized
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