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Invitrogen™ C. botulinum BoNT-A Light Chain Polyclonal Antibody
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Product Code. 15578542
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Quantity:
100 μg
Unit Size:
100µg
Product Code. Quantity unitSize
15578542 100 μg 100µg
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Product Code. 15578542

Brand: Invitrogen™ PA548053

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Description

Sheep Polyclonal Antibody

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant C. botulinum BoNTA Heavy Chain is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction. E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.
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Specifications

Antigen C. botulinum BoNT-A Light Chain
Applications Immunoprecipitation, Western Blot
Classification Polyclonal
Concentration 0.2 mg/mL
Conjugate Unconjugated
Formulation PBS with 5% trehalose and No Preservative
Gene Alias BONTC1
Host Species Sheep
Immunogen E. coli-derived recombinant Clostridium BoNT-A Light Chain Met1-Phe425.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Bacteria
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Lyophilized
Isotype IgG
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