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Thermo Scientific Chemicals BICINE, 0.5M buffer soln., pH 7.5

Product Code. 15443269
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Product Code. 15443269 Supplier Thermo Scientific Chemicals Supplier No. J60494.AK
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CAS: 150-25-4 | C6H12NNaO4 | 185.16 g/mol

A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

Solubility
Soluble in water (1M) at 20°C, ethanol (slightly), ether (slightly), and chloroform (slightly).

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 150-25-4
Molecular Formula C6H12NNaO4
Molecular Weight (g/mol) 185.16
MDL Number MFCD00004295
InChI Key MFBDBXAVPLFMNJ-UHFFFAOYSA-M
PubChem CID 8761
ChEBI CHEBI:40957
IUPAC Name 2-[bis(2-hydroxyethyl)amino]acetic acid
SMILES [Na+].OCCN(CCO)CC([O-])=O

Specifications

Concentration 0.5 M
pH 7.5
Physical Form Liquid
Quantity 250 mL
Solubility Information Soluble in water (1M) at 20°C,ethanol (slightly),ether (slightly),and chloroform (slightly).
Chemical Name or Material BICINE buffer soln.

RUO – Research Use Only

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