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Invitrogen™ beta-3 Tubulin Monoclonal Antibody (TU-20)
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Product Code. 11544542
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Quantity:
100 μg
Unit Size:
100µg
Product Code. Quantity unitSize
11544542 100 μg 100µg
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Product Code. 11544542

Brand: Invitrogen™ MA119187

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This item is not returnable. View return policy

Description

Mouse Monoclonal Antibody

This antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin. For immunohistochemistry applications, this product requires protein pretreatment (0.1% trypsin) in 0.1 M HCl; incubate for 30 minutes at room temperature. Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay. Western Blot: Reducing conditions.

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
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Specifications

Antigen beta-3 Tubulin
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone TU-20
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 15mM sodium azide; pH 7.4
Gene TUBB3
Gene Accession No. Q13509, Q4QRB4, Q9ERD7
Gene Alias 3200002H15Rik; beta 3 tubulin; beta tubulin 3; beta-4; betaIII-tubulin; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; I79_000194; LOC100341581; M(beta)3; M(beta)6; mc1R; melanocortin receptor 1; Neuron specific beta III Tubulin; neuron-specific class III beta-tubulin; TUBB; Tubb3; TUBB4; tubulin beta; tubulin beta 3 class III; Tubulin beta-3; tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4; Unknown (protein for MGC:134115)
Gene Symbols TUBB3
Host Species Mouse
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 10381, 22152, 246118
Target Species Human, Multi-species, Mouse, Rat
Content And Storage 4°C, do not freeze
Product Type Antibody
Form Liquid
Isotype IgG1
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