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  5. BD Pharmingen™ IHC Zinc Fixative

BD Pharmingen™ IHC Zinc Fixative

Product Code. 15886838
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Quantity:
500mL
1000mL
Dilution:
1 X
10 X
Unit Size:
Each
Product Code. Quantity Dilution unitSize
15886838 500mL 10 X Each
10400275 1000mL 1 X Each
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Product Code. 15886838

Brand: BD 552658

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Description

An alternative to formalin fixation of tissue for paraffin embedded sections

Ten percent neutral buffered formalin fixes tissue by cross-linking proteins, potentially causing a conformational change or “masking” of the antigenic epitope and leading to reduced or absent antibody reactivity. Different antigen retrieval techniques are used to unmask the epitope; however, many times it is not possible to retrieve the epitope and get optimal immunohistochemical staining. In these situations the use of IHC Zinc fixative, which is a milder fixative than formalin, is recommended.

  • Zinc-fixed paraffin embedded sections show very good immunostaining with many leukocytes markers and depict tissue morphology comparable to formalin-fixation
  • Use of antigen retrieval techniques on the zinc-fixed paraffin sections may further enhance the antibody reactivity in some cases

There are a few antigens that are not preserved by zinc-fixation. In these situations use of fresh frozen tissue for immunohistochemical staining is the only alternative.

TRUSTED_SUSTAINABILITY

Specifications

Boiling Point 100°C
Color Clear
Content And Storage Room temperature
Dilution 10 X
Product Type Fixative
Quantity 500mL
For Use With (Application) Immunohistochemical staining
pH 7.2
Form Liquid

Safety and Handling

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Product Identifier
  • 10X Zinc Fixative (Formalin Free)
 Signal Word
  • Danger
 Hazard Category
  • LONG-TERM AQUATIC HAZARD Chronic 2
  • Serious eye damage/eye irritation Category 1
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H411-Toxic to aquatic life with long lasting effects.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : zinc acetate, zinc chloride
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