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Bcl-X Monoclonal Antibody (2H12), Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen AHO0222
Additional Details : Weight : 0.50000kg
Description
This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. The immunogenic sequence (aa 3-14) is conserved in human and mice. Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. A recommended positive control is Hodgkin's lymphoma.
Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain that is well-conserved among members of the Bcl-2 family. This family of proteins is an important regulator of apoptosis. Bcl xL forms heterodimers with BAX, BAK, and Bcl2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl xL is phosphorylated on many sites including serine 62, a critical site for Bcl xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 is thought to be mediated by Jun N-terminal stress kinase (JNK) signaling - negatively regulates the anti-apoptotic function of Bcl xL and controls the growth of neoplastic cells.Specifications
Bcl-X | |
Monoclonal | |
0.2 mg/mL | |
BCL2L1 | |
Apoptosis regulator Bcl-X, BCL2L, BCL2L1, BCLX, BLC2L, Bcl-2-like 1 protein | |
Mouse | |
Protein A | |
RUO | |
12048, 24888, 397536, 598 | |
4° C | |
Liquid |
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot | |
2H12 | |
Unconjugated | |
Q07817, Q64373, O77737, P53563 | |
BCL2L1 | |
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS), of human Bcl-X protein. Thisamino acid sequence is shared by human and murine Bcl-X protein. | |
500 μL | |
Primary | |
Human, Mouse, Porcine, Rat | |
Antibody | |
IgG2a |