missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Reverse Transcription Products
  6. cDNA Synthesis Kits and Master Mixes
  7. Applied Biosystems™ TaqMan™ Reverse Transcription Reagents 

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

Product Code. p-4926541
200 Reactions at 10 μL, Each
Click to view available options
No. of Reactions:
200 Reactions at 10 μL
2000 Reactions at 10 μL
Unit Size:
1 set
Each
This item is not returnable. View return policy

Product Code. 10117254

Brand: Applied Biosystems™ N8080234

5142.64 NOK valid until 2025-12-31
BEST PRICE on Promo! Use promo code "25055" to get your promotional price.
This item is not returnable. View return policy

Description

Includes

MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents

  • Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
  • Includes random hexamers and oligo (dT)16 for flexibility
  • Individual components provide versatility in assay setup
  • Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents

  • TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
  • For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used
  • The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems

Specifications

Concentration 50 U/μL
Content And Storage

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
• RNase Inhibitor, 100 μL (20 U/μL)
• dNTP Mixture, 1 mL (2.5 mM each dNTP)
• Oligo (dT)16, 100 μL (50 μM)
• Random Hexamers, 100 μL (50 μM)
• 10X RT Buffer, 1.5 mL
• MgCl2 Solution, 1.5 mL

Store at –25°C to –15°C.
Format Kit
Technique Reverse Transcription
Optimal Reaction Temperature 42°C
Quantity 200 Reactions
Reverse Transcriptase MultiScribe
Ribonuclease H Activity Yes
Shipping Condition Dry Ice
For Use With (Application) Real Time PCR (qPCR)
Final Product Type First-Strand cDNA
No. of Reactions 200 Reactions at 10 μL
Reaction Format Separate components
Reagent Type Reverse Transcription
Size (Final Product) Up to 5 kb
Starting Material RNA
Show More Show Less

Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Product Code
Lot Number3118775 Certificate TypeCertificate of Analysis Date16/01/2025 Product CodeN8080234
Lot Number3028966 Certificate TypeCertificate of Analysis Date21/11/2024 Product CodeN8080234
Lot Number2964874 Certificate TypeCertificate of Analysis Date29/08/2024 Product CodeN8080234
Lot Number2924469 Certificate TypeCertificate of Analysis Date11/06/2024 Product CodeN8080234
Lot Number2901588 Certificate TypeCertificate of Analysis Date16/05/2024 Product CodeN8080234

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents >

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.